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Effect of remote ischemic preconditioning on hepatic parenchymal and microvascular damage in obesity
Author(s) -
O'Leary Heather A.,
Kothur Anand,
Fournier Sara B.,
Goodwill Adam G.,
Frisbee Jefferson C.,
Brock Robert W.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1117.9
Subject(s) - ischemic preconditioning , medicine , ischemia , population , cardiology , confounding , parenchyma , clamp , endocrinology , pathology , mechanical engineering , clamping , environmental health , engineering
Remote ischemic preconditioning (RIPC) is an adaptive response in which tolerance to prolonged periods of ischemia/reperfusion (I/R) is induced in a target organ by prior shorter periods of I/R in a distant organ. Although this phenomenon has been established within a healthy population, the effects of RIPC have yet to be defined within an obese population. The benefits coupled with this type of protection are of particular importance since obesity, and the confounding factors associated with it, are known to result in repeated ischemic events. Thus, we set forth to determine the effect of RIPC on direct hepatic I/R injury in ob/ob and db/db mice. For RIPC, we utilized a protocol of 10‐min of hindlimb ischemia followed by 10‐min of reperfusion repeated three times. Partial hepatic I/R (70%) was initiated by placing an atraumatic clamp across the portal triad between the quadrate and median/lateral lobes. Obese mice had significantly higher levels of liver parenchymal damage during I/R compared to controls (ob/ob: ~10‐fold & db/db: ~4‐fold). Sinusoidal endothelial cell (SEC) dysfunction also increased with I/R (ob/ob: ~2‐fold & db/db: ~4‐fold). After RIPC, the detrimental effects of hepatic I/R were improved by at least 60%, in both parenchymal damage and SEC dysfunction. Together, these data suggest that RIPC of the hindlimbs extends significant functional benefits to the ischemic livers of those with obesity.