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The Effect of Ethanol on Tracheal Smooth Muscle During Maturation
Author(s) -
Jakupaj Muharrem,
Sopi Ramadan B,
Krasniqi Avdyl,
Dreshaj Ismail A
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1115.5
Subject(s) - contraction (grammar) , nifedipine , ethanol , chemistry , muscle contraction , endocrinology , medicine , smooth muscle , biophysics , calcium , biology , biochemistry
In previous studies we have shown that ethanol induces contraction of pulmonary artery smooth muscle in dog. Ethanol induced also contraction of tracheal smooth muscle (TSM) in adult animals. However, the effect of ethanol during development is not well understood. Therefore, we hypothesize that ethanol induces contraction during whole postnatal life. The experiments were performed on TSM strips obtained form piglets and young pigs at three ages (3–7 days, 2–3 wk and 3 mo.). After anesthesia, the trachea was removed and tracheal strips were prepared, and placed in organ baths, filled with Krebs‐Henseleit solution to equilibrate. Tissues were challenged with different concentration of ethanol (46–276 mM). The role of extra‐cellular Ca 2+ was studied by incubation of tissues in KH solution with presence or absence of Ca 2+ or using a Ca 2+ channel blocker, nifedipine (10, 50, 100 μM). Ethanol induced concentration‐dependent contraction of TSM at three ages. The absence of Ca 2+ in extra‐cellular environment reduced TSM tone in response to ethanol. Also, blockade of Ca 2+ channels reduced contractile responses of TSM and the extent of this reduce depended from the concentration of nifedipine. Based on the results of this study we conclude that ethanol induces TSM contraction in piglets and this effect disclose since the birth. extra‐cellular Ca 2+ plays an important role on TSM tone in response to ethanol.