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Transient neonatal hypoxia alters adult ventilatory response, behavioral performance, and cortical capillary density in mice
Author(s) -
Tsipis Constantinos P,
Sun Xiaoyan,
Huang Pengjing,
Kikano Elias,
Radford Thomas,
Babcock Gerald,
Boron Walter,
LaManna Joseph,
Xu Kui
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1110.9
Subject(s) - hypoxia (environmental) , hypercapnia , hypoxic ventilatory response , medicine , endocrinology , anesthesia , chemistry , respiratory system , oxygen , organic chemistry
We investigated the effect of transient neonatal hypoxia on adult ventilatory response, behavioral performance and cortical microvascular density in wild‐type and knockout mice (NCBn‐1 KO, RPTP‐beta KO). Mice were exposed to either hypoxia (5% O2, 2 hrs) or normoxia at age P2. At P60, ventilatory response to hypoxia or hypercapnia (HVR, HCVR) and behavioral performance was measured. Parietal cortical capillary density was determined by GLUT‐1 immunohistochemistry following exposure to either normobaric hypoxia (8% O2) or normoxia for 3 weeks at P60. Results indicated a strain‐dependent difference in HVR, HCVR, and behavioral performance (cognitive, motor) amongst P2‐normoixc groups, while HVR in P2‐hypoxic mice was attenuated as compared to normoxic counterparts. NBCn‐1 KO P2‐hypoxic mice demonstrated improved cognitive and motor performance (Y‐maze, adhesion test) compared to the NBCn‐1 KO P2‐normoxic group. Baseline cortical capillary density (N/mm2) was significantly increased across all three strains in response to P2‐hypoxic exposure, but the hypoxic pretreatment had no effect on the magnitude of hypoxia‐induced brain angiogenesis. Our preliminary data suggest that transient neonatal hypoxic exposure appears to affect on adult ventilatory response, behavioral performance, and baseline cortical microvascular density.