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Reduced catalase and increased hydrogen peroxide contribute to the protective effect of chronic exercise against microvascular dysfunction induced by acute exertion
Author(s) -
Bian JingTan,
Goldufsky Josef,
Goslawski Melissa,
Franklin Nina C,
Phillips Shane A
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1108.3
Subject(s) - sed , medicine , endocrinology , catalase , leg press , exertion , endothelial dysfunction , aerobic exercise , chemistry , oxidative stress , resistance training
Previous studies indicate that hydrogen peroxide (H 2 O 2 ) scavenger catalase (CAT) reduced the protective effect of chronic exercise against endothelial dysfunction after acute exertion. We hypothesized that: 1) CAT protein is reduced after a single weight lifting (WL) session and 2) CAT expression is reduced in resistance compared to aerobic trained athletes. Gluteal fat biopsies were obtained in healthy, sedentary (SED), conditioned (EX, runners>15 miles/wk or WL>3x/wk), before and after a progressive leg press WL session. Resistance arteries (RA) from biopsies were cannulated for vascular reactivity measurements in response to acetylcholine (ACh; 10 −9 ‐10 −4 M). H 2 O 2 production and CAT protein were determined with DCF fluorescence and Western blot, respectively. All subjects had similar BP responses to WL. PEG‐CAT reduced ACh dilation and H 2 O 2 of EX subjects post‐ WL. CAT protein was reduced 45 ± 1% and 69 ± 1% in adipose and resistance arteries of EX subjects post‐ WL, respectively (n=12). The exercise‐induced reduction in CAT protein was more pronounced in conditioned WL (61%; pre: 1.5 ± 0.2 vs. post: 0.6 ± 0.2) compared to runners (39%; pre: 1.5 ± 0.5 vs. post: 0.9 ± 0.3) and SED (26%; pre: 1.1 ± 0.2 vs. post: 0.8 ± 0.1). These data indicate that reduced CAT protein following acute exertion may rise more pronounced in RA of trained athletes compared to sedentary subjects. NIH (K23HL085614).