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Nitric oxide but not prostaglandins is obligatory to the blood flow response during recovery following forearm exercise in humans
Author(s) -
Shepherd John Roger Alden,
Walker Branton G.,
Joyner Michael J.,
Casey Darren P.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1108.11
Subject(s) - ketorolac , forearm , medicine , blood flow , nitric oxide , brachial artery , anesthesia , blood pressure , surgery , analgesic
We tested the hypothesis that inhibition of prostaglandins and nitric oxide (NO) would have an additive effect on recovery of blood flow following rhythmic forearm exercise in 15 male subjects (26 ± 2 years). In separate protocols subjects performed forearm exercise (20% of maximum) for nine minutes. Forearm blood flow (FBF; ultrasound) and brachial artery pressure were measured at baseline, during exercise, and recovery. In protocol 1 (n = 7), exercise was repeated during cyclooxygenase (COX) inhibition (Ketorolac) alone and during Ketorolac/NOS inhibition (NG‐monomethyl‐L‐arginine; L‐NMMA). In protocol 2 (n = 8), exercise was repeated during L‐NMMA alone and during L‐NMMA/Ketorolac. Total FBF was calculated as the area under the curve over three minutes following exercise (recovery). Protocol 1: Recovery flow was not reduced by COX inhibition compared to control (468 ± 78 vs. 527 ± 74 ml; P = 0.18), but substantially attenuated during combined COX/NOS inhibition (290 ± 42; P < 0.01 vs. Ketorolac alone). Protocol 2: Recovery flow was attenuated with NOS inhibition alone compared to control (571 ± 165 vs. 829 ± 180 ml; P≤0.01), but not attenuated further during combined NOS/COX inhibition (540 ± 133 ml; P = 0.27 vs. L‐NMMA alone). These data indicate that NO but not prostaglandins contribute to blood flow recovery post forearm exercise. NIH HL‐46493 (MJJ), AR‐55819 (DPC) and by CTSA RR‐024150