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Leukocyte Release of IL‐6 in Response to Endotoxin in the Elderly: Effect of Aerobic Capacity
Author(s) -
Schneider Suzanne,
Dokladny Karol,
Mermier Christine,
Sood Akshay,
Sadek Joseph,
Moseley Pope
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1107.9
Subject(s) - peripheral blood mononuclear cell , immune system , morning , interleukin 6 , aerobic exercise , medicine , physical activity , treadmill , endocrinology , immunology , physiology , cytokine , chemistry , in vitro , physical therapy , biochemistry
Infection is a serious risk in the elderly, perhaps related to a decline in immune response. We hypothesized that aerobic capacity (VO 2 pk) and physical activity each would reduce leukocyte baseline IL6 release, while increasing IL6 release after endotoxin challenge. Men (n=6) and women (n=6), 61–87 yrs of age, rested overnight in a hospital and a fasting blood sample was obtained in the morning. Each subject completed a CHAMPS physical activity questionnaire to quantify weekly activity and a treadmill or cycle test to determine VO 2 pk. From each blood sample, PBMCs were isolated and cultured for 24 hrs in RPMI media at 37°C, 5% CO 2 . Media samples were obtained (time 0), then 10 ng/ml of endotoxin (Escherichia coli) were added to the cultures. The cells were again incubated and the media sampled after 2 hrs. The IL6 concentration from each sample was determined using ELISA. Neither total activity (kcal/wk) nor moderate activity (kcal/wk >3 METS) correlated with baseline IL6 or the stimulated increase in IL6. However, VO 2 pk was inversely correlated (r −0.58, P<0.05) with baseline, and positively correlated (r =0.58, P<0.05) with the normalized (2hr value/baseline value) increase in IL6. Our findings support the concept that maintaining aerobic fitness with aging may preserve immune acute phase response, although this benefit is more closely associated with VO 2 pk per se than with physical activity.