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Gene expression in different regions of rat tendons submitted to load exercise associated with nandrolone decanoate administration
Author(s) -
Cassia Marqueti Rita,
Heinemeier Katja M,
Durigan Joao Luiz Q,
Schjerling Peter,
Kjaer Michael,
Carvalho Hernandes Faustino,
SelistredeAraujo Heloisa Sobreiro
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1107.3
Subject(s) - tendon , ctgf , gene expression , medicine , anabolism , endocrinology , nandrolone , anatomy , gene , chemistry , growth factor , biochemistry , receptor
The use of anabolic androgenic steroids (AAS) increases risk for tendon injury, however, little is known about the effect of AAS in tendon gene expression. This study evaluated the expression of genes involved on tendon remodeling of the proximal and distal regions of calcaneal tendon, intermediate and distal region of superficial flexor tendon and proximal, intermediate and distal region of deep flexor tendon submitted to jumping water load exercise associated with AAS administration. Wistar male rats were grouped as follows: sedentary (S), trained (T), AAS‐treated (10 mg/kg/week) sedentary rats (AAS) and AAS‐treated and trained rats (AAST). mRNA levels of COL1A1, COL3A1, TIMP‐1, TIMP‐2, MMP‐2, IGF‐Ia, GAPDH, CTGF and TGF‐β were evaluated by real‐time PCR. Training did not alter the expression of COL1A1, COL3A1 and MMP‐2 genes, while AAS administration or its association with training reduced theses gene expression. IGF‐Ia enhanced with exercise, while AAS and AAST decreased their expression. In conclusion, the training did not alter the collagen expression and main growth factors in different regions of rat tendon. However, AAS or AAST showed harmful effects in the tendon ECM remodeling. The gene expression was distinct in each tendon region of sedentary animals, indicating that these regions possess a variance in expression of key genes responsible for the tendon adaptation. Grant from FAPESP‐06/50986‐6.