Premium
Effect of exercise training modality on skeletal muscle mitochondrial biogenesis in young and older adults
Author(s) -
Irving Brian A,
Lanza Ian,
Henderson Gregory,
Weymiller Audrey,
Schimke Jill,
Morse Dawn,
Nair K S
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1107.20
Subject(s) - tfam , mitochondrial biogenesis , skeletal muscle , medicine , endurance training , aerobic exercise , messenger rna , endocrinology , biology , mitochondrion , gene , genetics
The specific effects of different exercise modalities on mitochondria (mito) biogenesis are not fully understood. We determined the effects of endurance (ET), resistance (RT), and combined (CT) training on skeletal muscle mito biogenesis in young (Y) and older (O) adults. 33 young (25±1 y) and 27 older adults (70±2 y) were randomized to one of three 8wk interventions: (i) Control: activity levels unchanged, (ii) ET: cycling 60 min at 65% VO 2 peak, 5x/wk. (iii), RT: RT 4x/wk. After completing the control condition, the controls completed an 8wk (iv) CT program. Percutaneous muscle biopsies were acquired before intervention and 48h following the last training session. rtPCR was used to determine mtDNA and mRNA abundance of genes encoding transcriptional regulators of mito biogenesis (PGC1α, TFAM, NRF1, NRF2, SIRT1, SIRT3) with statistical analysis by ANOVA (mean (SEM)). ET and CT increased mtDNA in the O [63 (26)%, and 86 (35)%, p<0.05], but not Y. ET increased PGC1α [41 (20)%, 23 (14)%], NRF2 [60 (14)%, 22 (14)%], and SIRT3 [24 (9)%, 24 (9)%] mRNA abundance in the Y and O (P<0.05). CT also increased PGC1α [65.5 (26.3)%, 84 (50)%], TFAM [28 (13)%, 32 (23)%], and SIRT3 [24 (6)%, 35 (13)%] mRNA abundance in the Y and O (P<0.05). In conclusion, both ET and CT were effective at increasing mRNA abundance of genes encoding transcriptional regulators of mito biogenesis in both Y and O adults. Funding: R01AG09531, KL2RR084151, UL1RR24150