Premium
The influence of acetaminophen and exercise on rat Achilles tendon structural properties
Author(s) -
Whitt Jamie A,
Tedeschi J,
Gump B,
McMullan D,
Mundo J Del,
Moore M S,
Broderick T L,
Carroll C C
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1107.2
Subject(s) - hydroxyproline , placebo , tendon , achilles tendon , acetaminophen , treadmill , medicine , saline , tendinopathy , urology , surgery , pharmacology , pathology , alternative medicine
The purpose of this study was to evaluate the influence of acetaminophen (APAP) and exercise on the structural properties of rat Achilles tendon. Ten‐week‐old male Wistar rats were divided into placebo or APAP groups and further divided into sedentary or treadmill exercised groups. APAP (200 mg•kg −1 ) or placebo (saline) was administered once daily via oral gavage for 8 weeks. Tendon collagen content was determined by detection of the collagen specific amino acid hydroxyproline using high performance liquid chromatrography. Rats in the exercise group ran on a treadmill five days per week for 8 weeks with progression to 60 minutes per day, 20 m•min −1 , and 8° incline. Exercise did not influence tendon collagen content and data were collapsed into placebo (n=24) and APAP (n=26) groups. Achilles tendon collagen content was greater (p<0.065) in the rats given APAP (placebo: 598±22; APAP: 652±18 μg collagen•mg tendon wet weight). When expressed relative to tendon dry mass collagen content was not influenced by APAP (placebo: 1346±60; APAP: 1345±57 μg collagen•mg tendon dry weight). Evaluation of tendon water content revealed a 7% (p<0.01) decrease in tendon water content in the APAP group when compared to placebo. Our data suggests that chronic consumption of APAP results in a loss of tissue water from tendon and this effect may be independent of exercise. Midwestern University College of Health Science and Office of Research and Sponsored Programs