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Acute and chronic endurance exercise effects on nitric oxide, superoxide, and redox‐related gene expression in circulating CD34+ cells
Author(s) -
Jenkins Nathan T,
Landers Rian Q,
Prior Steven J,
Soni Naina,
Spangenburg Espen E,
Hagberg James M.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1107.16
Subject(s) - sod2 , enos , nitric oxide , superoxide , superoxide dismutase , medicine , endocrinology , nitric oxide synthase , chemistry , cd34 , intracellular , nadph oxidase , andrology , biology , oxidative stress , biochemistry , microbiology and biotechnology , stem cell , enzyme
The angiogenic potential of circulating CD34 + progenitor cells depends largely on the balance between intracellular nitric oxide (NO) and superoxide (O 2 − ) levels. Our objective was to determine intracellular NO and O 2 − levels and expression of redox‐related genes [endothelial nitric oxide synthase (eNOS), NADPH oxidase subunits p47 phox and gp91 phox , and superoxide dismutase‐2 (SOD2)] in CD34 + cells isolated before and after acute exercise in age‐ and BMI‐matched endurance‐trained (ET) and sedentary (S) men. ET had lower baseline CD34 + cell NO and O 2 − levels than S (P < 0.05), and baseline eNOS and p47 phox mRNA levels were higher in ET than S (P < 0.05). Acute exercise normalized group differences in NO and O 2 − , and p47 phox mRNA decreased significantly in both ET and S (P < 0.05). gp91 phox mRNA levels were similar between groups at baseline and decreased in S only (P < 0.05). We observed no differences in SOD2 mRNA levels between groups or with acute exercise. CONCLUSIONS The higher NO and lower p47 phox mRNA levels observed in the S group's CD34 + cells were unexpected, as our previous work indicated that exercise enhanced NO dynamics in circulating angiogenic cells. However, given the lower O 2 − levels and higher eNOS mRNA in the ET group's cells, it appears that exercise training enhances intracellular conditions for vasoprotective angiogenic actions of CD34 + cells.