Premium
Effects of exercise during pregnancy on lactate levels and monocarboxylate transporter expression in the rat
Author(s) -
Bauer Ashley Jean,
Chasson Sarah,
Banek Chris,
Johnson Britt K,
Gingery Anne,
Gilbert Jeffrey S
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1107.15
Subject(s) - medicine , endocrinology , pregnancy , skeletal muscle , amniotic fluid , basal (medicine) , physical exercise , fetus , placenta , biology , insulin , genetics
Although maternal blood lactate levels are increased by pregnancy and exercise, the regulation of MCTs in response to chronic exercise during pregnancy is unclear. Thus, we hypothesized 1) that exercise training results in decreased MCT1 and MCT4 expression in skeletal muscle; and 2) exercise training does not decrease amniotic fluid lactate concentrations. Female rats were given access to an exercise wheel for 6 weeks prior to mating and during pregnancy. On day 19 of pregnancy tissues were collected and snap frozen for later analysis. Western blots were performed in skeletal muscle and placenta. Resting blood lactate levels were increased in pregnant compared to non‐pregnant rats. Exercise training did not alter resting blood (2.5 ± 0.3 vs. 3.1 ± 0.3 mmol/l) or amniotic fluid (7.9 ± 0.5 vs. 7.4 ± 0.5 mmol/l) lactate concentrations in pregnant rats when compared to sedentary controls. Chronic exercise training during pregnancy decreased (P<0.05) maternal skeletal muscle MCT1 (2.6 ± 0.5 vs. 5.2 ± 0.7) and MCT4 (2.6 ± 0.8 vs. 7.1 ± 1.1) expression. Placental MCT1, 2 and 4 expression were not altered due to exercise. The present data indicate exercise training does not alter basal blood lactate levels or placental MCT 1 & 2 expression during late pregnancy. Down regulation of skeletal muscle MCTs 1 & 4 with exercise training before and during pregnancy may be an adaptive feature to maintain energy supply to the fetus.