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Interval and Continuous Exercise Regimens Suppress Hypoxia‐induced Thrombin Generation by Decreased Release of Procoagulant‐rich Microparticles from Neutrophils
Author(s) -
Chen YiChing
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1107.14
Subject(s) - thrombin generation , thrombin , tissue factor , hypoxia (environmental) , phosphatidylserine , flow cytometry , interval training , aerobic exercise , medicine , thrombosis , endocrinology , chemistry , immunology , coagulation , platelet , oxygen , biochemistry , organic chemistry , phospholipid , membrane
The release of procoagulant‐rich microparticles from neutrophils accelerates the pathogenesis of inflammatory thrombosis. This study explicates the manner in which interval and continuous exercise regimens affect neutrophil‐derived microparticles (NDMP) release and NDMP‐mediated thrombin generation (TG). Twenty sedentary males were randomly divided into moderate continuous exercise training group (MCT; 60% VO 2max , n=10) and aerobic interval exercise training group (AIT; 3‐minute intervals at 80% and 40% of VO 2max , n=10) for 30 minutes per day, 5 days per week for 5 weeks. The NDMP characteristics and dynamic TG parameters were measured by two‐color flow cytometry and calibrated, automatic thrombinography, respectively. Acute 12%O 2 exercise increased release of micrparticles from neutrophils and up‐regulated expression of phosphatidylserine (PS) on NDMP, which responses were accompanied by elevated thrombin peak height and increased TG rate in NDMP‐rich plasma. However, both MCT and AIT for 5 weeks decreased levels of tissue factor‐rich and PS‐exposed microparticles released from neutrophils and depressed NDMP‐mediated dynamic TG in plasma at rest and following the 12%O 2 exercise. Therefore, we conclude that both MCT and AIT ameliorates NDMP‐induced TG by down‐regulated expressions of pro‐coagulant factors on NDMP at performing 12%O 2 exercise, which may, in turn, decrease the risk of inflammatory thrombosis evoked by hypoxic stress.