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Effects of moderate intensity exercise training on the aortic eNOS‐Hsp90 interaction and insulin‐mediated vasorelaxation response in rats
Author(s) -
Campos Oscar Antonio,
Noble Earl G.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1106.1
Subject(s) - enos , insulin , medicine , endocrinology , hsp90 , intensity (physics) , chemistry , nitric oxide , nitric oxide synthase , biochemistry , heat shock protein , physics , quantum mechanics , gene
Exercise can improve insulin responsiveness and endothelial function in sedentary individuals, possibly through enhanced eNOS‐Hsp90 interaction. In this study, it was hypothesized that moderate intensity exercise training would result in greater insulin‐mediated vasorelaxation responses coupled with a greater eNOS‐Hsp90 interaction. Twenty male Sprague‐Dawley rats were randomly divided into exercise (E) and sedentary (S) groups. E rats walked on a treadmill at 15 m/min for 30 min, 4 d/wk, for 14–17 wks. Vasorelaxation responses to varying doses of insulin in excised aortic rings were analyzed. The eNOS‐Hsp90 interaction was analyzed in aorta homogenates via co‐immunoprecipitation. Insulin caused dose‐dependent vasorelaxation responses which were significantly lower in the E group compared to the S group at several insulin concentrations (2.4±2.2% vs 12.6±3.2% at 1.5 IU/mL; 4.8±2.9% vs 17.6±3.9% at 2.25 IU/mL; 9.2±2.9% vs 24.7±4.4% at 3 IU/mL, respectively, p<0.05). The level of eNOS and Hsp90 protein content as well as their interaction (1.5±0.3 vs 2.3±0.4, respectively, p=0.09) were not significantly different between the E and S groups. In summary, moderate intensity exercise training resulted in decreased insulin‐mediated vasorelaxation which was unlikely a consequence of changes in the eNOS‐Hsp90 interaction. Supported by CIHR Grant #CCT‐ 83029.