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Proteomic profile of patients before and after cardioplegia and cardiopulmonary bypass reveals specific changes to numerous metabolic and cytoskeletal proteins
Author(s) -
Clements Richard T,
Ivanov Alexander R,
Smejkal Gary,
Freeman Emily,
Bianchi Cesario,
Sellke Frank W
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1097.23
Subject(s) - cardiopulmonary bypass , cardioprotection , cytoskeleton , cardiac surgery , glycolysis , medicine , chemistry , cardiology , ischemia , biochemistry , metabolism , cell
cardiac surgery utilizing cardioplegia and cardiopulmonary bypass(CP/CPB) subjects myocardium to hypothermic reversible ischemic injury that impairs cardiac function, likely regulated via acute protein modifications. We determined changes in the protein profile of human myocardium following CP/CPB. Methods Right atrial appendage from patients pre and post‐CP/CPB (n=8), were subjected to 2D PAGE, staining and densitometry. Spots significantly altered pre or post CP/CPB were then excised and subjected to LC‐MS/MS. Results Pre‐CP/CPB, 32 unique or preferentially expressed (vs post‐CP/CPB) protein spots were detected. Post‐CP/CPB, 119 differential proteins (vs pre‐CP/CPB) were detected. 68 of the most prominent CP/CPB‐regulated spots were identified. CP/CPB caused prominent changes to multiple components of specific metabolic pathways, including numerous glycolytic and electron transport chain proteins. Other processes and or pathways enriched in the analysis included oxidative stress proteins and cytoskeletal constituents and regulators. Conclusions CP/CPB modifies specific metabolic, cytoskeletal, inflammatory, and proteolytic proteins potentially involved in deleterious side effects associated with cardiac surgery. Understanding global acute protein modifications in the heart may enhance therapeutic strategies for improved cardioprotection.