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Effect of rennin‐angiotensin system inhibition upon insulin secretion and superoxide production in pancreatic isolated islets of rats
Author(s) -
Batisti Fabricio Paula,
Haidar Andre Abou,
Quadros Caren D'Almora,
Rocha Marlene Santos,
Carpinelli Angelo Rafael,
Hirata Aparecida Emiko
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1095.3
Subject(s) - medicine , endocrinology , pancreatic islets , insulin , nad(p)h oxidase , secretagogue , chemistry , superoxide , nad+ kinase , secretion , islet , nadph oxidase , oxidative stress , biology , enzyme , biochemistry
Glucose is the main secretagogue of insulin, although several metabolizable energy substrates such as fatty acids and some cytokines may also influence the secretory response. Recently it was demonstrated that pancreatic islets express the enzyme NAD (P) H oxidase and is essential to the process of insulin secretion stimulated by glucose. Thus, the purpose of this study was to better understand the function and regulation of NAD (P) H oxidase of the organ. Rat pancreatic islets were isolated using collagenase. The isolated islets were incubated for 60 minutes in the presence of glucose, palmitate and leptin. NAD(P)H oxidase activation was measured using DHE method. Insulin secretion was evaluated by ELISA. Incubation of isolated pancreatic islets with palmitate caused a decrease in insulin secretion in the presence of 5.6mm‐glucose at the same time it promoted an increase in superoxide production. In terms of sub‐and supraphysiological glucose, palmitate did not alter insulin secretion, but led to a significant increase in ROS generation. Leptin increased the insulin secretion both under and above or under physiological glucose. It was impossible to correlate with the production of ROS.