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Fiber Type‐Selective Oxidative Damage and Myofiber Atrophy in the Gastrocnemius of Patients with Peripheral Arterial Disease
Author(s) -
Casale George P.,
Weiss Dustin J.,
Nella Aikaterini,
Swanson Stanley A.,
Zhu Zhen,
Miserlis Dimitrios,
Johanning Jason,
Pipinos Iraklis I.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1092.24
Subject(s) - myocyte , atrophy , oxidative stress , gastrocnemius muscle , medicine , oxidative phosphorylation , myopathy , muscle atrophy , pathology , cardiology , endocrinology , skeletal muscle , biology , biochemistry
Peripheral arterial disease (PAD) characterized by leg muscle ischemia due to atherosclerosis of leg arteries, affects ca. 10 million Americans. We demonstrated, previously, that a myopathy characterized by mitochondrial dysfunction and oxidative damage contributes to PAD pathophysiology. In this study, we evaluated the hypothesis that increased oxidative damage in the myofibers of gastrocnemius from PAD patients correlates with disease progression and is myofiber‐type selective. We found that oxidative damage was increased in PAD compared to control myofibers and correlated with clinical stage of disease and degree of blood flow limitation in the ischemic leg. Oxidative damage was higher in Type II and I/II fibers compared to Type I fibers. A disproportionate increase in lipid peroxidative damage was associated with increasing oxidative injury in the myofibers, pointing to greater damage to membrane systems. Mean cross‐sectional area of all myofiber types was significantly decreased in PAD gastrocnemius. These data demonstrate that increased oxidative damage in the myofibers of gastrocnemius from PAD patients correlates with disease progression, is myofiber‐type selective and is associated with myofiber atrophy.