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Temporal variation in NADPH oxidase expression occurs during successful collateral artery development
Author(s) -
Miller Steven J,
DiStasi Matthew R,
Gruionu Gabriel,
Chittajallu Nandita,
Turner Shelby,
Unthank Joseph L
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1092.18
The role of NADPH oxidase (Nox) in the promotion and impairment of collateral growth (CG) remains controversial. The aim of this study was to assess temporal expression changes of Nox isoforms and subunits in successful CG. An established model of ileal artery ligation and qPCR were used with young (2–3 mo) WKY rats to assess Nox expression during CG. Changes in collateral (high flow) artery Nox mRNA expression at 1 and 7 days relative to same‐animal normal flow control arteries are shown in the table. To assess the functional significance of transient elevated p47 phox expression in collaterals, hindlimb femoral artery ligations in p47 phox−/− and C57/BL6 (WT) mice were performed. Tissue perfusion (% control limb) at 14 days as assessed by LDPI showed impaired recovery in p47 phox−/− vs. WT (32±1.9% vs. 55±1.2%, respectively; p<0.05). Diameter measurements of collateral and control (gracilis) arteries at 14 days indicated significant collateral expansion in WT (76±7 vs. 33±4; p<0.01) but not p47 phox−/− (55±7 vs. 38±7; p=0.21). These results suggest that Nox isoforms/subunits, especially p47 phox , may mediate specific aspects of collateral development. Future studies will investigate the role of Nox in age‐related CG impairment. Support: HL092012