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High salt diet impairs angiogenic competence of bone marrow derived mononuclear cells
Author(s) -
Genthe Jamie Rae,
Stodola Tim,
Parker Sarah,
Greene Andrew S
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1091.1
Subject(s) - peripheral blood mononuclear cell , bone marrow , competence (human resources) , medicine , cancer research , chemistry , biochemistry , psychology , in vitro , social psychology
High salt diet suppresses the renin angiotensin system (RAS). Sprague Dawley (SD) rats fed high salt diet (HSD) show attenuated skeletal muscle angiogenesis. Exogenous delivery of bone marrow mononuclear cells (BM‐MCs) is a potential proangiogenic therapy; however more research is needed into how various disease states affect BM‐MCs function. We investigated the effect of RAS suppression by HSD on the ability of exogenous BM‐MCs to promote angiogenesis in SD recipient rats also on HSD. After one week on HSD (4% NaCl), 7–8 week old SD rats received 8 hours per day of electrical stimulation to their right hindlimb muscles for 7 days. One day prior to the onset of stimulation, vehicle or BM‐MCs derived from SD donor rats on HSD or normal salt (NSD) were injected intramuscularly into the stimulated limb. Microvessel density in muscles of the stimulated limb, relative to the unstimulated limb, was significantly higher in rats injected with BM‐MCs from a NSD donor. Vehicle and HSD BM‐MCs treated rats showed no angiogenic response to electrical stimulation. These results implicate the importance of a normal RAS in mediating BM‐MCs therapeutic competence.