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Time‐dependent effect of conjugated linoleic acid‐induced body fat loss and lipolysis in coconut oil fed mice
Author(s) -
Ippagunta Siri,
Barnes Kimberly M
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.109.2
Subject(s) - lipolysis , conjugated linoleic acid , perilipin , nefa , medicine , chemistry , endocrinology , coconut oil , adipose tissue , glycerol , fatty acid , linoleic acid , biochemistry , biology
Dietary conjugated linoleic acid (CLA) causes a body fat loss that is enhanced when mice are fed coconut oil (CO). The objective was to determine if there is a time‐dependent effect of CLA feeding on lipolysis. Male mice (ICR; n=80; 3wk‐old) were fed 7% SO or CO diets for 6wk then 0 or 0.5% CLA for 3, 7, 10 or 14d. A body fat index (BFI) was calculated and lipolysis was determined ex vivo by NEFA and glycerol release from adipose tissue. The relative expression of perilipin and phosphorylated perilipin (P‐perilipin) were determined by western blotting. The BFI was reduced by CO on d7 (P<0.01), and by both CLA (P<0.05) and CO (P<0.05) on d14. NEFA release was increased by CLA in CO‐fed mice (1.84 vs 7.93 μmol/g; P<0.01) but not in SO‐fed mice (1.64 vs 2.03 μmol/g) on d7 but on d14 CLA increased NEFA release in both CO (2.83 vs 6.16 μmol/g) and SO‐fed mice (2.01 vs 4.99 μmol/g). Glycerol release was increased by CLA in CO‐fed mice but not in SO‐fed mice on d3 and d7 (P<0.05). P‐perilipin was not altered by diet but total perilipin tended to be increased by CLA in CO‐fed mice (P=0.055) on d7. Therefore CLA‐induced lipolysis may occur more rapidly in CO vs SO‐fed mice but appears to be transitory.