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Ameliorative effects of proanthocyanidin on renal ischemia/reperfusion injury
Author(s) -
Yanarates Omer,
Guven Ahmet,
Sizlan Ali,
Uysal Bulent,
Akgul Emin Ozgur,
Atim Abdulkadir,
Ozcan Ayhan,
Korkmaz Ahmet,
Kurt Ercan
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1087.3
Subject(s) - glutathione peroxidase , malondialdehyde , kidney , blood urea nitrogen , oxidative stress , creatinine , chemistry , antioxidant , superoxide dismutase , ischemia , renal ischemia , reperfusion injury , endocrinology , medicine , pharmacology , biochemistry
NTRODUCTION Several natural products have been reported to have beneficial effects on ischemia/reperfusion (I/R) injury, particularly from a preventative perspective. Therefore, this study was designed to investigate the efficiency of proanthocyanidin (PA), a natural product derived from grape seed, on renal dysfunction and injury induced by I/R of rat kidney. MATERIALS AND METHODS Twenty‐four male Sprague‐Dawley rats were divided into three groups: sham‐operated, I/R, I/R+PA. Rats were given PA (100 mg/kg/day peroral) 7 days prior to I/R. All rats except sham‐operated underwent 60 min of bilateral renal ischemia followed by 6 h of reperfusion. After reperfusion, kidneys and blood were obtained for evaluation. Superoxide dismutase, glutathione peroxidase, malondialdehyde, protein carbonyl content, and nitrite/nitrate level (NO(x)) were determined in the renal tissue. Serum creatinine (S(Cr)), blood urea nitrogen (BUN), and aspartate aminotransferase (AST) were determined in the blood. Additionally, renal sections were used for histological grade of renal injury. RESULTS PA significantly reduced the I/R‐induced increases in S(Cr), BUN, and AST. In addition, PA markedly reduced elevated oxidative stress product, restored decreased antioxidant enzymes, and attenuated histological alterations. Moreover, PA attenuated the tissue NO(x), levels indicating reduced NO production. CONCLUSIONS The pretreatment of rats with PA reduced the renal dysfunction and morphological changes, ameliorated cellular injury, and restored renal antioxidant enzymes caused by renal I/R.