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Studies of age‐related impairments in regenerative capacity in adult mammals using the rodent bladder
Author(s) -
Burmeister David,
AbouShwareb Tamer,
D'Souza Sean,
Herco Maja,
Andersson KarlErik,
Christ George
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1087.13
Subject(s) - rodent , rodent model , regeneration (biology) , biology , neuroscience , physiology , ecology , microbiology and biotechnology , endocrinology
Although models of organ regeneration in lower vertebrates (e.g., urodeles) have been utilized to identify mechanisms involved in regeneration, more relevant models in animals closer to humans phylogenetically are lacking. We previously reported on a rodent model of bladder regeneration, where removal of ~70% of the bladder (subtotal cystectomy; STC) induces a regenerative response in 12 week old female rats, such that normal volume and function are restored within 8 weeks. Herein we report on the impact of age on the remodeling/regenerative response of the bladder after STC in 12 month old females. Cystometric studies revealed that bladder capacity 8 weeks post‐STC was 60% of age‐matched controls, and maximum pressures generated in bladders 8 weeks post‐STC (33.3 ± 4.5 cmH2O) were also low compared to controls (56.7 ± 4.8cmH2O). H&E staining of excised kidneys revealed damage consistent with hydronephrosis in STC animals, and was accompanied by a significant increase in kidney weight (STC animals: 1.10 +/− 0.03; controls: 0.99+/− 0.02g). These data clearly demonstrate an age‐related decline in regenerative capacity of the bladder, potentially leading to end stage renal disease. As such, the rodent bladder may provide mechanistic insight on the salient features of impaired regeneration, which may lead to new therapeutic possibilities for tissue repair/regeneration. This research supported by R21DK081832.