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Baseline serum cardiac troponin I concentrations in various strains of rats as determined with an ultrasensitive immunoassay
Author(s) -
Herman Eugene,
Knapton Alan,
Rosen Elliot,
Lu QuynhAhn,
Estis Joel,
Agee Sara,
Todd John,
Lipshultz Steven,
Zhang Jun
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1086.3
Subject(s) - troponin i , medicine , troponin complex , ovariectomized rat , immunoassay , endocrinology , troponin , cardiology , myocardial infarction , estrogen , antibody , immunology
Cardiac troponins (cTnT and I) are reliable biomarkers for detecting myocardial injury. New ultrasensitive assays have been able to detect baseline serum cTn levels even in healthy patients. Recently, an ultrasensitive cTnI immunoassay (Erenna IA, Singulex, CA) has detected increases from baseline cTnI levels due to drug‐induced myocardial injury in rats, dogs and monkeys and baseline cTnI ranges in Sprague‐Dawley (SD) rats. The present study was initiated to use the Erenna cTnI assay to further document baseline cTnI levels in normal control animals from multiple strains, including spontaneous hypertensive (SHR), SD, Wistar, Wistar‐Kyoto, Fisher, SHR ovariectomized and SHR castrated rats. Baseline cTnI concentrations were detected in all 115 rats tested. Male rats had higher mean cTnI concentrations than females of the same strain.(SHR 26 vs 10 pg/ml) SHR male rats had the highest baseline levels (mean=26 pg/ml and the largest cTnI variability(3.9–70.2 pg/ml). Increased cTnI levels were observed for castrated SHR compared to unaltered male SHR, (51 vs 16 pg/ml) while ovariectomized SHR had lower cTnI concentrations than normal female SHR (mean=4 vs 9 pg/ml).. These results show quantifiable differences in cTnI concentrations between strains, sexes and non‐cardiac surgical alterations in control animals. Thus, there is a continuing need to develop an expanded knowledge base of control information in order to realize maximal potential from monitoring small changes in cardiac troponin by new ultrasensitive assays.