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Angiotensin II induction of thirst requires neuropeptide W
Author(s) -
Pate Alicia Thurman,
Samson Willis Kendrick
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1079.18
Subject(s) - thirst , angiotensin ii , endogeny , medicine , endocrinology , chemistry , receptor , neuropeptide , renin–angiotensin system , neuropeptide y receptor , vasopressin , water intake , food intake , blood pressure
Neuropeptide W (NPW), an endogenous ligand for G protein‐coupled receptors NPBWR1 (GPR7) and NPBWR2 (GPR8), is present in hypothalamic nuclei known to be important in food and water intake (Ann NY Acad Sci 1200:162,2010), and is thought to play an important role in the regulation of feeding and drinking behavior, and the neuroendocrine response to stress (Am J Physiol 288:R270,2005). In rat, central administration of NPW increases food intake in the light phase, but decreases food intake during the dark phase (Br J Pharmacol 148:1033,2006). We hypothesized that endogenous NPW was a physiologically relevant participant in the CNS regulation of thirst and therefore pretreated male rats with siRNA against NPW or vehicle intracerebroventricularly (i.c.v.) once daily for two days. siRNA treatment did not significantly alter body weight in these animals. On the third day, we administered a dipsogenic dose of angiotensin II (50 pmole, i.c.v.) and monitored water intake in normally hydrated animals. NPW siRNA pretreatment significantly reduced angiotensin II‐induced water drinking during the first 2 hr following angiotensin II administration; however, cumulative water intakes at 24 hr post angiotensin II challenge did not differ between groups. Our results suggest that endogenous NPW contributes to the neuronal activation of thirst in response to angiotensin II. Supported by HL 066023.