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Reactive oxygen species production and modulation of rhythmogenesis from VRG neurons
Author(s) -
Garcia Alfredo J,
Dean Jay B,
Ramirez Jan Marino
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1074.3
Subject(s) - production (economics) , reactive oxygen species , biology , microbiology and biotechnology , economics , macroeconomics
We have previously demonstrated that both catalase and hydrogen peroxide (H 2 O 2 ) biphasically change the frequency of rhythmicity from the preBötzinger complex (preBötC). This ongoing study tests a two‐fold hypothesis: (1) neurons of ventral respiratory group (VRG) at the level of the preBötC generate intracellular reactive oxygen species (ROS); (2) the effects of H 2 O 2 are unique to the H 2 O 2 and cannot be mimicked by generalized oxidative stress. In vitro imaging and electrophysiological experiments were performed from neonatal rodent slices (600μm; CD1 mice or Spague Dawley rats; P4–11). Intracelllular ROS imaging using either dihydrorhodamine 123 or dihydroethidium illustrate heterogeneous production of H 2 O 2 or its precursor, superoxide anion, from VRG neurons. This heterogeneous ROS production occurs during control, hypoxia, and reoxygenation. Moreover, extracellular recordings show that neither co‐application of Fe 2+ and H 2 O 2 to produce hydroxyl radical nor the chemical oxidant chloramine T could mimic the effect of H 2 O 2 alone on rhythmogenesis in the preBötC. These findings suggest that endogenous production of ROS may centrally modulate inspiratory rhythmogenesis. Supported by NIH Grants