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State‐Dependence of Ventilation (VE) and Neuromodulator Concentration at the Pre‐Bötzinger Complex (preBötC) in Response to Cholinergic Receptor Blockade
Author(s) -
Muere Clarissa,
Miller Justin,
Neumueller Suzanne,
Hodges Matthew R.,
Forster Hubert V.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1074.1
Subject(s) - cholinergic , non rapid eye movement sleep , medicine , endocrinology , blockade , atropine , chemistry , muscarinic acetylcholine receptor , excitatory postsynaptic potential , neuroscience , anesthesia , receptor , biology , eye movement
Cholinergic neurons provide excitatory input to the postulated site of respiratory rhythm generation, the preBötC [ J Neurophysiol 83: 1243–1252, 2000]. Cholinergic activity is decreased in NREM sleep compared to the awake state [ Respir Physiol Neurobiol 131: 135–144, 2002]. Thus, cholinergic receptor blockade at this site should have a greater effect on V E in the awake state compared to NREM. To test this hypothesis, we chronically cannulated 2 adult goats at a site just dorsal to the preBötC for microdialysis (MD) of mock cerebrospinal fluid (mCSF) alone or in combination with 50mM atropine (AT), a muscarinic acetylcholine receptor antagonist. Effluent dialysate was collected for quantification of neuromodulators. MD of mCSF alone in the day or night had no effect on V E in either the awake or NREM state. MD of AT in the day increased V E . MD of AT at night had inconsistent effects in the awake state, but decreased V E in NREM. MD of AT resulted in increased serotonin levels in effluent mCSF from 1.5 to 2.8 and 5.8 nM in the day and night, respectively. These preliminary data are inconsistent with the stated hypothesis and suggest that the V E response to cholinergic receptor blockade is state‐ and circadian rhythm‐dependent and affected by compensatory changes in other neuromodulators. Supported by NIH HL25739 and Veterans Administration