Premium
Age dependent changes in A 2A receptor expression in PreBotzinger Complex
Author(s) -
Mayer Catherine A.,
Foglyano Ryan,
Wilson Christopher G.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1073.4
Subject(s) - neun , stereology , receptor , in vivo , medicine , respiratory system , endocrinology , receptor expression , adenosine a2a receptor , biology , pathology , immunohistochemistry , adenosine receptor , agonist , microbiology and biotechnology
Adenosine is a modulator of respiratory output, and can both increase and decrease respiratory frequency in an age and receptor dependent manner. We have shown an in vivo , age dependent, effect of A 2A receptor activation on apneas in rats which is attenuated from postnatal day (P)14 to P21 through adulthood. We hypothesized that the attenuated response to A 2A activation was due to decreased A 2A receptor expression in the PreBötzinger complex (pBc). To test this hypothesis, we used unbiased stereology to quantify changes in A 2A expression in pBc over the first three weeks of life. Sprague Dawley rat pups were perfused at P0, 4, 7, 14, and 21. The brains were cryosectioned and 4 identical sets of slides made from each brain. These slides were immunostained for NK‐1R and somatostatin to deliniate the pBc. The remaining slides were stained with A 2A , NeuN or S100β antibodies. The number of immunopositive cells in the pBc and the pBc volume were quantified using unbiased stereology (n=5 for each antibody at each age). The number of cells immunopositive for A 2A per 1X10 5 μm 3 of pBc decreased significantly between P0 and P7 (p < 0.006), and rose again by P21 (P < 0.2). The number of NeuN and S100β immunopositive cells per 1X10 5 μm 3 decreased significantly between P0 and P21 (p < 0.001 for both). We conclude that A 2A expression changes with age, but these changes are not correlated with the pattern of A 2A dependent apneas seen in vivo.