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The effect of prenatal androgen receptor blockade on breathing development in neonatal male and female rats
Author(s) -
Wenninger Julie M,
Holley Heidi S,
Behan Mary
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1073.1
Subject(s) - medicine , apnea , flutamide , endocrinology , testosterone (patch) , ventilation (architecture) , respiratory distress , plethysmograph , androgen , respiratory system , hypoxia (environmental) , androgen receptor , anesthesia , hormone , chemistry , prostate cancer , mechanical engineering , cancer , engineering , organic chemistry , oxygen
There are sex biases in the occurrence of respiratory disorders including Obstructive Sleep Apnea (OSA) and Sudden Infant Death Syndrome (SIDS). In neonatal rats, there are changes in respiratory control during the early neonatal period. Postnatal day 12–13 (P12–13) appears to be a “sensitive period” in breathing development in rats. However, little is known about the sex differences at this age and the effects of testosterone in breathing development. The purpose of our study was to eliminate the pre and perinatal testosterone surges and measure eupneic ventilation and the hypoxic ventilatory response (HVR) in awake neonatal rats from P10–15, at puberty (~P30) and in young adult (P90) male and female rats. Pregnant dams were given daily subcutaneous injections of the androgen antagonist flutamide (20mg/kg) from G14 until delivery. At P10‐P15, P30 and P90 rats were studied in a whole‐body flow‐through plethysmograph and breathing and blood O 2 saturation were continuously monitored in room‐air and in hypoxia (12% O 2 ). Following plethysmography, each rat was anesthetized and arterial blood collected for hormone measurement. Animals were perfused and the carotid bodies and brainstems harvested for immunohistochemical analysis. Flutamide treatment significantly changed O 2 saturation in P30 and P90 female but not male rats. Supported by NIH AG18760 and the Parker B Francis Foundation.