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Pituitary Adenylate Cyclase‐Activating Peptide (PACAP) and Substance P (SP) induce the release of Brain‐Derived Neurotropic Factor (BDNF) from the longitudinal muscle layer of the intestine
Author(s) -
Alqudah Mohammad,
Mahavadi Sunila,
Zachary Bradley L.,
Kay Jarren C.,
Murthy Karnam S,
Grider John R
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1070.4
Subject(s) - medicine , endocrinology , neuropeptide , secretion , substance p , neurotrophic factors , western blot , brain derived neurotrophic factor , pituitary adenylate cyclase activating peptide , chemistry , vasoactive intestinal peptide , biology , receptor , biochemistry , gene
BDNF has been implicated in neuronal development and plasticity. While BDNF is expressed in neural and non‐neuronal cells, the expression and secretion of BDNF in gastrointestinal smooth muscle is not well defined. Aim To determine if BDNF is expressed and secreted from intestinal smooth muscle. Methods BDNF expression in intestinal segments was evaluated immunohistochemically. Longitudinal muscle cells were cultured for 24 to 48 hours in the absence or presence of SP or PACAP (10 to 100 nM); BDNF content in cells and secretion into media was measured by western blot and ELISA. Results Immunohistochemically, BDNF was identified primarily in the longitudinal rather than circular muscle layer of mouse and rabbit intestine. Western blot analysis of BDNF from longitudinal muscle cultures confirmed basal BDNF expression and demonstrated time‐ and concentration‐dependent increase in BDNF content in response to both neuropeptides, with PACAP being more effective than SP. Similar results were obtained for release of BDNF into culture medium. Conclusion In the basal state, intestinal smooth muscle cells from the longitudinal layer express and secrete BDNF. Contractile and relaxant neuropeptides modulate this expression and secretion suggesting crosstalk between enteric neurons and smooth muscle via interplay between neuropeptide transmitters and neurotrophins. Supported by NIDDKD.