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Continuous Nitric Oxide Suppression of Lipolysis in Ambulatory Children
Author(s) -
Hickner Robert Charles,
Geyer Gabriel,
Squibb Ryan C.,
Mazzawi Jeanette,
Tanner Charles
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1062.11
Subject(s) - lipolysis , microdialysis , medicine , endocrinology , nitric oxide , saline , glycerol , in vivo , chemistry , adipose tissue , biochemistry , biology , microbiology and biotechnology , central nervous system
Increased nitric oxide (NO) may result in reduced lipolysis in obese adults, as has been determined in the laboratory setting. The extent to which suppression of lipolysis by NO occurs in free‐living children has not been determined, despite the rapid rise in incidence of childhood obesity. Purpose To 1) confirm that there is a greater suppression of lipolysis in obese than lean children, and 2) determine if there is a persistent inhibition of lipolysis by NO in the free‐living condition. Methods In‐vivo lipolysis was measured over 24 hrs via microdialysis of subcutaneous abdominal fat (SCAF) in 54 obese and 31 lean 8–11 yr old children. Two microdialysis probes were inserted into SCAF and perfused with either: 1) control solution (Saline), or 2) 10 mg/ml L‐NMMA (nitric oxide synthase inhibitor). RESULTS Fasting, resting dialysate glycerol (index of lipolysis) was lower in obese (61.5±4.0μM) than lean (88.6±7.4 μM) children (P<0.05). Dialysate glycerol was inversely related to BMI %tile (r 2 =.261). Fasting, resting dialysate glycerol was lower in probes perfused with saline (71.4±3.8 μM) than with L‐NMMA (87.4±4.9 μM; P<0.05), which persisted over the 24 hrs. Conclusion In‐vivo lipolysis is lower in obese than lean children. Furthermore, there is a persistent suppression of in‐vivo lipolysis by NO in children. The extent to which these conditions contribute to, or are a reflection of, the obese state is yet to be determined. Supported by NIDDK: 1R01DK071081