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Estradiol treatment, physical activity, and muscle function in mice that have experienced natural or chemically‐induced ovarian failure
Author(s) -
Greising Sarah M,
Kosir Allison M,
Lowe Dawn A
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1060.3
Subject(s) - isometric exercise , ovariectomized rat , endocrinology , medicine , soleus muscle , contractility , skeletal muscle , ovary , premature ovarian failure , estrous cycle , senescence , hormone
Estradiol (E 2 ) treatment in young adult ovariectomized mice reverses deleterious effects on muscle and also increases physical activity. The aim of this project was to determine if similar effects occur with E 2 treatment in mice with intact but failed ovaries. Wheel running and daily cage activities were monitored for ~55 days before mice were euthanized and soleus muscles analyzed for contractility. Aged, 24‐mo‐old, ovarian‐failed mice that were treated with E 2 wheel ran 2‐fold less than aged‐matched untreated mice (p=0.03), with no differences in cage activities such as ambulation distance and jump counts (p≥0.55) or in soleus muscle maximal isometric force (p≥0.39). Because E 2 treatment did not improve muscle function or physical activity in the aged, ovarian‐failed mice as predicted, a second study was initiated. 3‐mo‐old mice were treated with 4‐vinylcyclohexene diepoxide (VCD) to cause premature senescence of the ovaries, resulting in young, ovarian‐failed mice. VCD‐treated mice wheel ran and engaged in cage activities to the same extent as control and VCD+E 2 mice (P≥0.18), indicating that the presence of the ovaries is important for physical activity whether or not ovarian hormones are being produced. Further analysis of skeletal muscle contractility following VCD treatment may begin to explain the effects of aging and E 2 on muscle function. Supported by NIH grant R01‐AG031743.