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Effects of Green Tea and EGCG on IL‐8 and NF‐κB Inflammatory Responses in LPS‐Induced, Fully Differentiated HT‐29 Human Colon Cancer Cells
Author(s) -
Story Erica N,
Jordan Chad C,
Harris Gabriel K
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.106.1
Subject(s) - nf κb , green tea , cancer research , cancer , colorectal cancer , chemistry , medicine , inflammation , food science
Consumption of green tea (GT) is associated with reduced colon cancer incidence. Colon cancer has been linked to inflammation and diet. Epigallocatechin‐3‐gallate (EGCG), the main flavonoid component of GT, as well as GT itself, have demonstrated anti‐inflammatory and cancer preventive effects in numerous model systems. The purpose of this research was to determine the effects of GT or EGCG on IL‐8 formation and NF‐κB expression in lipopolysaccharide (LPS)‐induced human colon cancer (HT‐29) cells. Aqueous GT and EGCG extracts were prepared and matched for total phenol content by the Folin‐Ciocalteu method. Cell viability was determined by the MTT assay. Differentiation was induced by sodium butyrate and confirmed by the alkaline phosphatase assay. Cells were co‐treated with LPS 055:B5 (1 ng/ml) and three concentrations of GT or EGCG (0.0045, 0.009 and 0.045 mg/ml) for 24 hours. Interleukin 8 (IL‐8) concentration was measured by ELISA. Nuclear Factor‐κB (NF‐κB) concentration was examined by Western Blot. Neither GT or EGCG were toxic at the levels used. Although GT and EGCG showed trends toward decreases in IL‐8 formation and NF‐κB expression, only the highest (0.045 mg/ml) EGCG treatment was significant (p < 0.05). EGCG, but not GT, demonstrated anti‐inflammatory effects in this cell line, suggesting potential benefits relative to human colon cancer. This project was supported by the state of North Carolina.