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Increased insulin sensitivity and insulin signaling protein content following regular exercise in T1‐diabetic rats
Author(s) -
Hall Katherine E,
Noble Earl G,
Melling CW James
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1057.13
Subject(s) - glut4 , medicine , endocrinology , insulin , glucose uptake , insulin receptor , glucose transporter , insulin resistance , chemistry
Type 1 diabetic (T1D) patients can develop insulin insensitivity due to the daily requirement of exogenous insulin. Regular exercise has been shown to improve insulin sensitivity (IS) through the increased expression of muscle Glut4 and insulin receptor (IR) content. This study examined the role of exercise on IS and Glut4 and IR expression in T1D rats. Thirty animals were randomly divided into 3 groups; control (C), diabetic sedentary (D), and diabetic exercised (DE). D and DE were injected with STZ and implanted with insulin pellets to maintain blood glucose levels between 9 and 15 mM. DE ran on a treadmill at 27m/min, 1hr/day, 5days/wk for 10wks. Compared to C, D demonstrate a significant decrease in Glut4 and IR protein content in the red (RV) and white (WV) vastus muscles (p<0.05), and a significant reduction in blood glucose clearance following an intravenous glucose challenge (p<0.05). DE demonstrated a significant increase in Glut4 protein in RV and WV (p<0.05), while IR protein content was elevated in the WV only (p<0.05). Also, blood glucose clearance rates were significantly increased in DE compared to D (p<0.05). To conclude, STZ‐diabetic rodents exhibit insulin insensitivity which can be alleviated through regular exercise. These improvements in IS though exercise are concomitant with changes in Glut4 and IR protein content in a muscle‐specific fashion. Supported by CIHR Grant #CCT‐ 83029.