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Expression of MnSOD, CuZnSOD and catalase in response to chronic environmental hyperthermia in pigs
Author(s) -
Selsby Joshua T,
Johnson Kirsten,
GardanSalmon Delphine,
Hollinger Katrin,
Nearing Marie,
Rhoads Robert,
Lonergan Steven,
Gabler Nicholas,
Pearce Sarah,
Baumgard Lance
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1052.6
Subject(s) - catalase , gene expression , medicine , antioxidant , endocrinology , hyperthermia , skeletal muscle , biology , superoxide dismutase , andrology , oxidative stress , chemistry , gene , biochemistry
Chronic environmental hyperthermia (CEH) has a profound negative effect on mammalian health and performance. The study objective was to determine the extent to which CEH alters antioxidant enzyme expression and free radical production in muscle. We hypothesized that CEH would increase expression of MnSOD, CuZnSOD, and catalase independent of decreased caloric intake. Crossbred gilts (35±4 kg BW; n=6/group) were housed in thermoneutral (TN; 20°C) or heat stress (35°C) conditions for 1 d (HS1), 3 d (HS3), or 7 d (HS7). We also included a TN group that was pair‐fed to the HS7 group (TNPF). Pigs were sacrificed following 1, 3, or 7 days of controlled environmental exposure and the red (R) and white (W) semitendinosus (ST) removed. MnSOD gene expression increased 45 and 40% in R and W ST, respectively, and catalase increased 2.3 fold in RST in the HS1 compared to TN. In contrast, antioxidant expression in HS3 and HS7 was similar to or lower than TN in both muscle types. Protein expression mirrored gene expression data, though tended to lag one time point behind. This compensatory failure is due, at least in part, to the reduced caloric intake associated with CEH as the TNPF group had similar or lower expression of CuZnSOD and MnSOD compared to the TN and HS7 groups. These data indicate that CEH alters the expression and abundance of skeletal muscle antioxidant enzymes and that nutritional status may have a significant impact on this effect.