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Effect of fatty acids on avian satellite cell proliferation, differentiation and heparan sulfate proteoglycan expression
Author(s) -
McFarland Douglas Craig,
Velleman Sandra G.,
Pesall Jane E.,
Coy Cynthia S.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1051.43
Subject(s) - linoleic acid , syndecan 1 , oleic acid , arachidonic acid , chemistry , polyunsaturated fatty acid , cell growth , biochemistry , proteoglycan , fatty acid , biology , cell , extracellular matrix , enzyme
The objective of this study was to determine the effects of fatty acids on the proliferation, differentiation, and expression of syndecan‐4 and glypican‐1 in avian myogenic satellite cells (SC). SC derived from the pectoralis major (PM) and biceps femoris (BF) muscles of the turkey and chicken were individually administered 8 different fatty acids in defined medium during proliferation. A parallel set of turkey SC were induced to differentiate. As 5 micromolar linoleic acid exhibited maximal proliferation, this level was used in subsequent treatments. Highest levels of proliferation of turkey PM and BF SC occurred in cultures containing oleic acid. Linoleic acid and oleic acid were equipotent in supporting proliferation of chicken SC. Microscopic examination revealed that inclusion of DHA or EPA was toxic towards both PM and BF SC from both species. Linolenic acid and arachidonic acid diminished levels of differentiation. Expression of glypican‐1 varied between treatments to a greater extent with turkey BF than with PM SC. Expression in chicken PM and BF SC demonstrated a similar pattern in response to treatments. Turkey PM syndecan‐4 expression varied between treatments, whereas expression in turkey BF SC was similar between treatments. Expression in chicken SC varied little between treatments. The results demonstrate species and muscle‐specific differences in the parameters examined. It is proposed that changes in lipid raft receptor interactions may contribute to these observed differences. Support by the Midwest Poultry Research Program.