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hsp70 mRNA localization in rodent myofibers early post heat and exercise stress
Author(s) -
Silver Jordan Thomas,
Noble Earl G.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1050.5
Subject(s) - hsp70 , messenger rna , in situ hybridization , heat shock protein , biology , cytoprotection , microbiology and biotechnology , heat shock , translation (biology) , andrology , endocrinology , medicine , biochemistry , gene , oxidative stress
Rapid translation of the survival transcript, inducible heat shock protein 70 ( hsp70 ), is critical for mounting cytoprotection against severe cellular stress in myofibers. Housekeeping transcripts are thought to reside throughout the myofiber, but localization of hsp70 mRNA remains unclear. The purpose of this investigation was to examine the localization of hsp70 mRNA in myofibers post heat and exercise stress. Rats were divided into three groups (n=5): control (CON); heat stress (HS – 42°C for 15 min); and acute exercise (EX – 30 m/min, 6% grade, 1 h run). in situ hybridization of hsp70 mRNA was examined in mixed (MIX) and fast (FAST) skeletal muscles 1 h post‐treatment. In MIX‐CON, constitutive hsp70 mRNA was localized near nuclei. This nuclear localization was not altered by HS or EX in both MIX & FAST despite significantly elevated hsp70 mRNA expression as compared to CON; (P<0.05). HS and EX promoted globalized and differential hsp70 mRNA expression patterning respectively amongst the myofibers of MIX & FAST; (P<0.05). Results support that hsp70 mRNA may be translated at the site of nuclear export in stressed myofibers, which may contribute to rapid protein expression post‐insult. Supported by CIHR Grant #CCT‐ 83029 and NSERC #8170‐05 .