Premium
Skeletal tissues in tilapia express the extracellular calcium‐sensing receptor
Author(s) -
Loretz Christopher A.,
Pollina Catherine,
Herberger Amanda L.,
Hyodo Susumu,
Takei Yoshio
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1048.1
Subject(s) - chordate , vertebrate , biology , calcium sensing receptor , g protein coupled receptor , mineralized tissues , receptor , axial skeleton , anatomy , microbiology and biotechnology , medicine , gene , calcium , pathology , genetics , calcium metabolism , dentin
Molecular phylogenetic analysis suggests that the extracellular calcium‐sensing receptor (CaSR) emerged evolutionarily from an ancestral Class C G protein‐coupled receptor (GPCR) related to pheromone and vomeronasal receptors. CaSRs are unknown among non‐chordate animals, suggesting an association of CaSRs with the chordate‐vertebrate lineage. Our studies overall explore the evolution of CaSRs, and the possible historical linkage of CaSRs to vertebrate skeleton as functional components of calcium homeostasis through storage and/or release. We applied both RT‐PCR and immunohistochemistry to evaluate Casr gene and CaSR protein expression, respectively, in skeletal tissues of a cichlid teleost, the Mozambique tilapia ( Oreochromis mossambicus ). By RT‐PCR, Casr gene expression was observed in skull and vertebrae (including notochordal tissues). Immunochemistry revealed CaSR protein expression in chondrocytes within hyaline cartilage, and in notochordal sheath cells within the vertebral canal. Observation of bone‐associated CaSR immunoreactivity was more difficult, as a consequence, perhaps, of the acellular nature of bone in this advanced bony fish. Casr may be part of a genetic network associated with vertebrate skeletal system. Supported by Japan Society for the Promotion of Science (YT and SH), University of Tokyo AORI Visiting Professorship (CAL), and National Science Foundation (ALH).