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Adiponectin receptor‐transgenic mesenchymal stem cells are more competent to differentiate into adipocytes
Author(s) -
Lin YuanYu,
Liu BingHsien,
Lin Yun,
Chiu YaoPang,
Wu ShinnChih,
Chen ChingYi,
Mersmann Harry John,
Ding ShihTorng
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1043.6
Subject(s) - adipogenesis , mesenchymal stem cell , adipocyte , microbiology and biotechnology , coactivator , transcription factor , biology , adipokine , adiponectin , endocrinology , stem cell , medicine , adipose tissue , insulin resistance , insulin , biochemistry , gene
Previously studies showed that adipogenesis in the bone marrow is positively associated with osteoporosis. To evaluate the roles of adiponectin, an energy homeostasis‐associated adipokine, and its receptors (AdipoR1 and R2) in modulating the cell fate between adipocytes and osteoblasts, we used the AdipoR1‐ and R2‐transgenic mice to establish their transgenic mesenchymal stem cells (R1/2‐tMSCs). Microarray analysis revealed that as compared to the wild‐type, the expression of adipogenic transcription factors, CCAAT/enhancer‐binding protein‐α and β and PPAR‐γ coactivator 1‐α was significantly higher, whereas the osteogenic transcription factors, msh homeobox2 and Wnt10b, was significantly lower, in R1/2‐tMSCs. Furthermore, R1‐tMSCs were more competent toward adipocyte lineage than normal MSCs. Taken together, our results suggest that adiponectin and its receptors upregulate the adipocyte differentiation‐associated transcription factors and thereby promote the differentiation of MSCs into adipocytes. Identification of the molecular mechanisms modulating the balance between osteogenesis and adipogenesis will further contribute to the understanding and treatment of metabolic‐linked bone diseases. This work is supported by a grant of Excellent Research Projects from National Taiwan University. HJM is a member for ASBMB and ANS.