Premium
Identification of the proteomes of c‐kit and Sca‐1 expressing populations of mice cardiac stem cells
Author(s) -
Gomes Adriana,
Costa Gonçalo,
Cordeiro Carlos,
Matsuda Alex Jun,
Fonseca AnaMarta,
Rosario Luis Bras
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1043.13
Subject(s) - stem cell , biology , microbiology and biotechnology , cell sorting , chemistry , flow cytometry
Cardiac Stem Cells(CSCs) are a promising approach for regeneration therapy. For this purpose it is essential to identify the proteins involved in the mechanisms of mobilization, differentiation and proliferation. Using the stem cell markers Sca‐1 or c‐kit, two CSCs populations were isolated by Fluorescence Activated Cell Sorting. Proteins were identified by Matrix‐Assisted Laser Desorption Ionization‐Fourier Transform Ion Cyclotron. We identified 119 proteins in c‐kit+ and Sca‐1+ CSCs, including: 2 proteins previously uncharacterized(C2orf18, LP9056); proteins involved in proliferation control(Protein Chibby Homolog1); myeloid related protein(S100‐A8), suggesting a possible hematopoietic origin; and proteins involved in chemotactic reactions(Myristoylated Alanin‐Rich C kinase substrate and Calgranulin‐A). We also identified proteins specific of c‐kit+ CSCs(Alpha Enolase); or of the Sca‐1+ CSCs(47 kDa heat shock protein) and Sortilin, a protein involved in differentiation. Proteomic analysis identified novel pathways that characterize the phenotype and function of CSCs and that will be the target of functional characterization and possible therapeutic applications