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Extract from Poria cocos on hypertonic stress‐induced water channel expression
Author(s) -
Lee So Min,
Lee Yun Jung,
Hwang Sun Mi,
Yoon Jung Joo,
Kang Dae Gill,
Lee Ho Sub
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1041.43
Subject(s) - tonicity , stress (linguistics) , expression (computer science) , chemistry , computer science , biochemistry , philosophy , linguistics , programming language
A major physiological role of the kidney is to regulate body water and urine concentration. Aquaporin‐2 (AQP2), a family of water channels, plays an important role in the urinary concentrating process and regulation of water balance in the kidney. The dried sclerotia of Poria cocos Wolf (WPC) is a well‐known Oriental medicinal plant that grows around the roots of pine trees. WPC has been known to have a diuretic effect and used for the treatment of chronic diabetic and nephrosis. Experiments were performed in mouse inner medullary collecting duct (mIMCD‐3) cell line. Hypertonic stress was applied with hypertonic (NaCl 175 mM) solution. We confirmed hypertonicity‐induced increased in AQP2 expression. WPC (10 – 50 mg/ml) attenuated the hypertonicity‐induced protein and mRNA levels of AQP2 in a concentration‐dependent manner. It identified expression of SMIT to verify through the agency of transcription factor tonicity‐responsive enhancer binding protein (TonEBP). Hypertonicity increased the levels of TonEBP and SMIT mRNA. WPC attenuated the hypertonicity‐induced TonEBP and SMIT levels. Similaly, WPC attenuated the hypertonicity‐induced phosphorylated‐SGK, but not SGK. Furthermore, hypertonicity increased cAMP levels and the changes were blocked by WPC. Taken together, the present data suggest that WPC inhibits hypertonicity‐induced AQP2 expression via SGK, TonEBP and cAMP signaling pathway.