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The Dopamine D1‐like Receptors Interact with the α1A Adrenergic Receptor in Human Renal Epithelial Tubule Cells
Author(s) -
Ennis Riley Charles,
Villar Van Anthony M,
Jurgens Julie A,
Jones John Edward,
Jose Pedro A
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1041.16
Subject(s) - receptor , proximal tubule , dopamine receptor , d1 like receptor , microbiology and biotechnology , alpha 1b adrenergic receptor , dopamine , adrenergic receptor , dopamine receptor d1 , chemistry , endocrinology , renal tubule , medicine , biology , kidney , beta 3 adrenergic receptor
As a multifactorial disorder, essential hypertension is influenced not just by the individual's lifestyle and environment but also by the interplay of a myriad of physiologic systems that either promote or protect against sustained increase in blood pressure. The D1‐like dopamine receptors (D 1 R and D 5 R) and the α 1A adrenergic receptor (ARα 1A ) are endogenously expressed in the kidney and promote contrasting effects on sodium transport, resulting in natriuresis (D 1 R and D 5 R) or anti‐natriuresis (ARα 1A ). We tested the hypothesis that the D 1 R and D 5 R interact with ARα 1A in human renal proximal tubule cells (hPTCs). We found that D 1 R and D 5 R co‐immunoprecipitated endogenous ARα 1A and pulled‐down heterologous ARα 1A ‐myc,His and that treatment with the D1‐like receptor agonist fenoldopam (1μM/10 min) enhanced the interaction. D 1 R and D 5 R also colocalized with ARα 1A in HEK293 cells, in which fenoldopam increased their colocalization at the perinuclear area, and in proximal tubules (and distal tubules for D 5 R) in human kidney sections. Prolonged (24‐hr) fenoldopam treatment resulted in decreased D 1 R (70.0±5.9% vs. 100±3.7%, P<0.05, n=3) and D 5 R (50.1±10.7% vs. 100±10.7%, P<0.05, n=3) and increased ARα 1A abundance (142.6±4.3% vs. 100±6.4%, P<0.05, n=3) in hPTCs, suggesting that the D1‐like dopamine receptors may regulate the expression of ARα 1A . Understanding the interaction between these receptors is essential to comprehending the crosstalk between anti‐ and pro‐hypertensive systems.