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Bioavailability of Human Milk Derived Peptides Using A Cell Culture Model
Author(s) -
Friel James,
DiehlJones William
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.104.7
Subject(s) - chemistry , peptide , trolox , bioavailability , antioxidant , pepsin , amino acid , chromatography , biochemistry , linoleic acid , emulsion , food science , biology , antioxidant capacity , pharmacology , enzyme , fatty acid
Human milk (HM) contains many known antioxidants some of which may be bioactive peptides. To identify novel peptides HM pepsin and pancreatin digests were separated using molecular membranes and reverse phase chromatography. The ORAC antioxidant assay yielded a peptide fraction (PF‐23) with high antioxidant activity (5207 μmoles Trolox Equivalents (TE)/g). Tandem MS identified 8 small molecular weight peptides from 4 to 6 amino acids, which were synthesized and screened using ORAC and linoleic acid emulsion. YGYTGA (5169 μM TE/mmol) and ISELGW (4479 μM TE/mmol) were found to be the most active as well as having antimicrobial and anti‐inflammatory properties. These two peptides were studied for transport across the gut using Transwell inserts seeded with 10 5 CaCo2 cells per well. Cells were exposed to 50, 100 and 200 ug/mL peptides for 3 hours with buffer collected from both apical and basal compartments. Teer values for each confluent well (>500) indicated an intact cell layer. No peptides were identified in control wells and approximately 10% of YGYTGA at 200 ug/mL was measured in the basal compartment and 1% ISELGW at all concentrations. These results may be relevant to infant formula fortification for the premature infant. Supported by AFMNet and MICH.