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Lipidomic analysis reveals differential lipid profiles and novel lipid species of interest in morbidly obese subjects compared to lean
Author(s) -
Donovan Elise L.,
Lewis Matthew R.,
Hamilton Karyn L.,
Miller Benjamin F.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1037.1
Subject(s) - dyslipidemia , medicine , endocrinology , lipid profile , obesity , oxidative stress , lipid metabolism , chemistry , lipidomics , biochemistry , cholesterol
Obesity is associated with co‐morbidities including coronary artery disease (CAD), and is characterized by conditions including oxidative stress and dyslipidemia. The combination of dyslipidemia and oxidative stress leads to increased oxidized lipids, which have been implicated in obesity associated CAD. The purpose of this study was to use a shotgun and targeted lipidomic analysis to examine lipid profiles and oxidation of lipids in morbidly obese bariatric patients compared to lean controls. Orthogonal partial least‐squares discriminant analysis (OPLS‐DA) revealed distinct separation of obese and lean groups based on plasma lipid profiles. Further analyses of the ions driving that separation, chromatograms, and mass spectrum data led to 42 lipid species that are significantly different between lean and obese subjects and contribute to the group separation. Interestingly, the oxidized phospholipids best characterized in the literature as playing a role in CAD do not appear significantly different in lean and obese subject plasma. These results suggest that while there is a clear difference in lipidomic profiles in obese subjects compared to lean, the difference may be due to lipid species whose role has yet to be examined. It is important to determine why these lipid species are different in morbidly obese subjects, and what potential pathogenic roles they may have as this could have clinical implications.