Premium
Enzyme activity and heart failure I: do lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) isozyme activities in several organs reflect pathophysiological alterations in heart failure
Author(s) -
BlandoHoegler Charlene F,
Hoegler Carl S
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1033.18
Subject(s) - malate dehydrogenase , lactate dehydrogenase , isozyme , ventricle , enzyme , heart failure , biology , dehydrogenase , biochemistry , medicine , endocrinology
A recent review (Stanley et al ., 2005) suggested that differential protein expression in various tissues in early stages of heart failure (HF) may contribute to symptoms. The present study investigates two enzymes, lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) to determine whether their activities show parallel changes in different tissues in control and end‐stage HF dogs. LDH isozyme activities in skeletal muscle (SM), aorta (AO) and right atrium (HA) showed alterations in the banding pattern [on polyacrylamide gel electrophoresis (PAGE)] in HF animals compared to controls. Overall, LDH activity in left ventricle (HV) was comparable in both control and HF animals, though there is one less isozyme band in the HF animal. In contrast, MDH isozyme activities in HF tissues (SM, AO, HA, HV) appear to be similar in band composition and intensities to control tissues. These differential effects may reflect a subtle interplay of metabolic activities in HF organs. The present study also implies that physiological changes in end‐stage HF may be due to or be a consequence of metabolic alterations in a number of organs. This work has received partial support from a Pace Scholarly Research grant.