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Alterations in O‐linked‐β‐N‐acetylglucosamine levels during acute exercise
Author(s) -
Porter Karen,
Marsh Susan A
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1033.12
Subject(s) - cardioprotection , cytosol , sed , chemistry , reperfusion injury , cardiac function curve , medicine , physical exercise , endocrinology , ischemia , enzyme , biochemistry , heart failure
Acute bouts of exercise reduce damage to cardiomyocytes during ischemic events, including a reduction in infarct size and improvement in heart function. The mechanism underlying exercise‐induced cardioprotection is still unknown. Previous reports have demonstrated increases in O‐linked‐β‐N‐aceytlglucosamine (O‐GlcNAc) provide protection against ischemia‐reperfusion injury, yet the role of O‐GlcNAc in exercise has not been investigated. Our lab proposes O‐GlcNAc may be a major factor in exercise‐induced cardioprotection. We predicted an increase in O‐GlcNAc levels in both cellular and nuclear compartments of male CD‐1 mouse hearts following acute exercise (EX) compared to a sedentary control (SED). O‐GlcNAc levels were not altered in either cytosolic or nuclear fractions of the 30 min EX group and SED group. O‐GlcNAc transferase (OGT), the enzyme that attaches O‐GlcNAc to proteins, was not different between 30 min groups. The 15 min groups did not demonstrate a difference in cytosolic levels of OGT, however, there was a significant decrease in OGT levels in the nuclear compartment of the EX group. Overall, the data suggest that cardiac O‐ GlcNAc and OGT levels either do not change or decrease during acute exercise.