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Hydrogen sulfide dilates cerebral arterioles by activating smooth muscle cell plasma membrane K ATP channels
Author(s) -
Liang Guohua,
Adebiyi Adebowale,
McNally Elizabeth M.,
Leffler Charles W.,
Jaggar Jonathan H.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1026.8
Subject(s) - glibenclamide , vasodilation , cerebral arteries , sulfonylurea receptor , arteriole , vascular smooth muscle , potassium channel , patch clamp , medicine , chemistry , endocrinology , biophysics , microcirculation , electrophysiology , anatomy , biology , smooth muscle , diabetes mellitus
Hydrogen sulfide (H 2 S) is a gaseous signaling molecule that appears to contribute to the regulation of vascular tone and blood pressure. Multiple potential mechanisms of vascular regulation by H 2 S exist. Here, we tested the hypothesis that H 2 S dilates piglet cerebral arterioles by activating ATP‐sensitive K + (K ATP ) channels in smooth muscle cells. Patch‐clamp electrophysiology indicated that Na 2 S or NaHS, two different H 2 S donors, activate K + currents in isolated cerebral arteriole smooth muscle cells. H 2 S‐induced K + current activation was partially reversed by glibenclamide, a selective K ATP channel inhibitor. H 2 S donors dilated isolated pressurized (40 mmHg) piglet cerebral arterioles, and this vasodilation was partially reversed by glibenclamide. Vasoregulation by H 2 S was also studied in resistance‐size cerebral arteries of mice with a targeted deletion in the gene encoding sulfonylurea receptor 2 (SUR2 null mice), a K ATP channel subunit. H 2 S‐induced vasodilation was smaller in pressurized cerebral arteries of SUR2 null mice than in wild‐type controls. These data indicate that H 2 S dilates cerebral arterioles of newborn piglets and cerebral arteries of mice by activating arterial smooth muscle cell K ATP channels and that SUR2 subunits are necessary for this functional effect.