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The effects of short term exercise training on purinergic metabolism in skeletal muscle arterioles
Author(s) -
Stone Audrey J,
Evanson Kirk W,
Kluess Heidi A
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1026.24
Subject(s) - adenosine , endurance training , chemistry , medicine , treadmill , purinergic receptor , endocrinology , sed , 5' nucleotidase , inosine , zoology , biology
The purpose was to determine the effects of short term exercise training on purinergic metabolism in gastrocnemius first‐order arterioles of young female rats. Female rats (2–3mo) were randomly assigned to one of the following four groups: endurance training (ExTr), sedentary for 14 days (SED), no exercise training or sedentary period (BSLN), and exercise trained followed by deconditioning (DECON). The exercise training consisted of running on a treadmill 5 days/week for 2 weeks at 35m/min and on a 7% incline. Adenine nucleotidase activity was measured using whole vessel segments in a spectrophotometric assay. Adenosine (ADO) overflow was measured with a biosensor sensitive to adenosine and inosine. Adenine nucleotidase activity was not different among the groups (BSLN: 0.17±0.08μM phosphate/μg protein, n=12; SED: 0.36±0.11 μM phosphate/μg protein, n=12; ExTr: 0.50±0.14 μM phosphate/μg protein, n=11; DECON: 0.23±0.06 μM phosphate/μg protein, n=13; p>0.01). Average adenosine overflow was highest in the ExTr group (324.9±323 μM ADO, n=4) and lowest in the DECON group (12.23±5.6 μM ADO, n=5; p>0.01 different from all other groups). The pattern of adenosine overflow also differs with exercise training and time. This study suggests that purinergic metabolism does alter with short term exercise training and detraining. This research was supported by the Arkansas Biosciences Institute and the National Institute on Aging.