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Signs of aging‐related inflammation and adaptive reserves of aged mesenteric lymphatic vessels
Author(s) -
Gashev Anatoliy A.,
Nagai Takashi,
Akl Tony,
Thangaswamy Sangeetha,
Chatterjee Victor,
Cote Gerard,
Bridenbaugh Eric A.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1022.5
Subject(s) - lymphatic system , inflammation , contractility , nitric oxide , nitric oxide synthase , ex vivo , homeostasis , systemic inflammation , in vivo , medicine , biology , pathology , immunology , microbiology and biotechnology
Adequate adaptation of mesenteric lymphatic vessels (MLV) to various volumetric loads is vital for whole‐body fluid/macromolecule homeostasis, lipid adsorption, and immunity. Such adaptive reserves are greatly diminished in aged MLV due to severe alterations in their stretch‐ and shear‐induced contractile responses. We recently found signs of shear‐independent, NO‐dependent depletion of contractility in MLV isolated from adult (9 mo old) and aged (24 mo old) F‐344 rats. Subsequent experiments with high‐speed imaging of aged MLV in vivo provided additional evidence of significant aging‐induced contractile depression in this lymphatic network. Results of experiments with non‐selective nitric oxide synthase (NOS) inhibition by L‐NAME, performed both in and ex vivo, support the idea of constant activation of inducible NOS (iNOS) in aged MLV. These findings correlate with elevated immunohistochemical labeling of iNOS in aged MLV and with the biochemical signs of a chronic inflammatory environment in and around of aged lymphatic wall. Cumulatively, these findings demonstrate a link between age‐related inflammation and functional disturbances in aged MLV, which leads to their diminished ability to adapt effectively to normal and potential abnormal volumetric challenges in elderly. NIH RO1 AG030578, HL094269.