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EFFECT OF PROPOLIS WITH TOPICAL ADMINISTRATION ON ACID‐INDUCED COLITIS IN RATS
Author(s) -
Ozturk Haluk,
Surer Ilhami,
Surer Hatice,
Demirbag Suzi,
Guven Ahmet,
Atabek Cuneyt,
Caliskan Bahadir
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1018.10
Subject(s) - propolis , enema , saline , colitis , malondialdehyde , acetic acid , medicine , irrigation , antioxidant , gastroenterology , pharmacology , chemistry , traditional medicine , biochemistry , biology , agronomy
PURPOSE Propolis, which is a specific product of honeybee hive exhibits potential antioxidant and anti‐inflammatory properties. This study was designed to determine the effects of propolis on acid‐induced experimental colitis model in rats. METHODS The experimental acid induced colitis was performed in 56 Wistar‐Albino rats according to Mann and Demers. Then rats were divided into two equal groups (Sl‐saline irrigation and Pl‐propolis irrigation). After preliminary saline irrigation in all rats either propolis (1.5 ml–100 mg/kg) or saline enema (1.5 ml) was administered by rectal route after acetic acid induction/In both groups irrigation procedure was performed in every 12 hours until colonic resection time. At 3rd – 7th – 10th and 14th post operative days rats were killed and distal colonic segments were harvested to determine the histopathological changes and tissue levels of malondialdehyde (MDA). RESULTS Severity of the mucosal injury in Pl group was significantly lower when compared with the Sl group. Additionally MDA levels in Sl group was higher statistically significant than Pl group. COCLUSION Topical administration of propolis as a rectal enema has significantly improved rectal mucosal restoration rate and both histopathological parameters and MDA levels on acid‐induced colitis model.