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3D‐based analyses of pharmacological modulation of zebrafish locomotion
Author(s) -
Cachat Jonathan,
Reihl Russell,
Cosgrove Jessica,
Gaikwad Siddharth,
Green Jeremy,
Hart Peter,
Vollmer Karoly,
Utterback Eli,
Kyzar Evan,
Rhymes Kate,
Hook Molly,
Chang Katie,
Stewart Adam,
Newman Alan,
Allain Alex,
Pham Mimi,
Kalueff Allan
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1006.1
Subject(s) - zebrafish , anxiolytic , novelty , neuroscience , psychology , anxiety , pharmacology , medicine , biology , psychiatry , social psychology , biochemistry , gene
Here, we integrated manual observations, computerized event‐based scoring and automated video analysis of zebrafish traces to generate three‐dimensional (3D) swim path reconstruction of general behavioral patterns, thereby improving the characterization and interpretation of complex, anxiety‐related adult zebrafish behavior. Drugs known to evoke a high‐anxiety state (caffeine, repeated morphine withdrawal) or an anxiolytic‐like effect (ethanol, morphine, fluoxetine, nicotine) wereused to detect multiple anxiety‐like behaviors and correlate them to those established manual observations. Additionally, we used this method to characterize the effects of lysergic acid diethylamide and 3,4‐methylenedioxymethamphetamine on zebrafish novelty‐evoked exploration. Our approach markedly enhances the power of zebrafish behavioral pharmacology, by providing innovative techniques for high‐throughput, multidimensional neurophenotyping of adult zebrafish behavior.