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Distribution of Intravenously Administered Gold Nanoparticles at the Light Microscope Level
Author(s) -
Haines Diana C,
Stern Stephen,
Hall Jennifer,
Patri Anil,
McNeil Scott
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1002.6
Subject(s) - lamina propria , submucosa , pathology , pigment , medicine , lymph node , lymph , chemistry , bone marrow , epithelium , organic chemistry
Female mice received an experimental anti‐cancer therapeutic conjugated to a PEGylated gold nanoparticle (GNP) via tail vein either alone, or in combination with ip docetaxel (DTX). Mice were sacrificed at day 10 and 17. Mice receiving the higher doses of GNP had discolored or grey tissues which correlated, microscopically, with a dose‐related “pigment”. At the light microscope level, the greatest concentration was noted in hepatic Kupffer cells, splenic red pulp macrophages and lymph node sinusoidal macrophages. Mild to moderate accumulation was present in the uterine endometrial stoma, renal glomeruli, adrenal cortex and medulla, gastrointestinal lamina propria and submucosa, and bone marrow. Minimal pigment, usually vascular associated, was also noted in other organs. There were no pigment‐associated lesions. The material was interpreted to be aggregated gold nanoparticles and was visible on unstained sections. The recovery group (17‐day) showed decrease in DTX‐associated lesions; however, the GNP‐associated pigment persisted. Increased “severity” of pigment in some organs in the recovery group was due to localized clumping of pigment aggregates. Funded by NCI Contract No. HHSN261200800001E